Sensitive and precise. Use samples as small as 5 μL. Linear detection range 0.08 mg / dL (20 μM) to 20 mg / dL (5 mM) Ca2 + in 96-well plate assay.
Simple and high performance. The procedure involves the addition of a single working reagent and incubation for 3 min. It can be easily automated as a high throughput assay for thousands of samples per day.
Improved stability and versatility of reagents. The optimized formulation has greatly improved signal and reagent stability. 96-well plate or cuvette assay.
Low interference in biological samples. No pretreatments are needed. Assays can be performed directly on raw biological samples, that is, in the presence of lipids, proteins, and minerals such as magnesium, iron, and zinc.
CALCIUM is measured to control bone diseases or disorders of calcium regulation. Elevated serum calcium levels are reported in hyperparathyroidism, metastatic bone lesions, and hypervitaminosis, while reduced levels are seen in hypoparathyroidism, nephrosis, rickets, steatorrhea, nephritis, and calcium loss syndromes.
Urine calcium levels help the doctor understand how the kidneys handle calcium in certain diseases of the parathyroid gland. Urinary calcium levels are also essential in the medical evaluation of kidney stones. Simple, straightforward, automation-ready procedures for measuring calcium concentration in biological samples are becoming popular in drug discovery and research.
The BioAssay Systems Calcium Test Kit is designed to measure calcium directly in biological samples without any pretreatment. A phenolsulfonphthalein dye in the kit forms a very stable blue complex specifically with free calcium. The intensity of the color, measured at 612 nm, is directly proportional to the concentration of calcium in the sample. The optimized formulation minimizes any interference from substances such as magnesium, lipids, proteins, and bilirubin.