Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 8 pg/ml
  • Range: 39 pg/ml – 2500 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay Type: Sandwich (quantitative)
  • Reacts with: Human

The Human PSA ELISA Kit (ab264615) is a 90-minute single wash sandwich ELISA designed for the quantitative measurement of PSA protein in EDTA plasma, hep plasma, serum, fetal calf serum, urine, and cit plasma. It uses our patented SimpleStep ELISA technology. Quantify human PSA with a sensitivity of 8 pg/ml.

SimpleStep ELISA technology employs capture antibodies conjugated with an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA plates. This sandwich ELISA approach allows for the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the simple steps ELISA protocol summary in the imaging section for more details. Our SimpleStep ELISA technology offers several benefits:

– Single wash protocol reduces assay time to 90 minutes or less
– High sensitivity, specificity, and reproducibility of superior antibodies
– Fully validated in biological samples
– 96-well plate that can be broken into 12 x 8-well strips

A 384-well SimpleStep ELISA microplate (ab203359) is available for use as an alternative to the 96-well microplate provided with the SimpleStep ELISA kits.


Prostate-specific antigen (PSA) or also commonly called tissue kallikrein-3 (KLK3) is a 34 kD serine protease of the kallikrein family. PSA has a very high expression in the prostate gland, but the expression in the breast and saliva has also been reported. PSA works by hydrolyzing Semenogelin-1 which leads to the liquefaction of the seminal clot. This is essential for the proper release of sperm during ejaculation.

The majority (> 70%) of PSA is known to form a complex with alpha-1-antitrypsin (PSA-ACT) in the blood. The remaining PSA exists as an inactive, unbound enzyme known as free PSA. PSA is best characterized as a clinical biomarker for prostate cancer. During the progression of prostate cancer, excessive amounts of PSA leak into the blood, dramatically raising the levels of total and free PSA. This assay detects total and free PSA levels.

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